INTERFERENCE ON HbA1C MEASUREMENT BY HPLC: HB D
Mehmet Kalaycı, Hakan Ayyıldız, Mahmut Bozkurt, Mustafa TimurkaanABSTRACT
A 59-year-old diabetic male patient applied our laboratory for HbA1C and routine test measurements. The patient’s HbA1C value could not be measured in our device. Upon examining his HbA1C chromatogram, abnormal band was detected in the patient. We took sample from the patient again and sent it for hemoglobin electrophoresis. In the end, hemoglobin D (HbD) was detected.
Although HbA1C measurement by HPLC is accepted as gold standard, hemoglobin variant can cause method-based interference in HbA1C measurements. Recognising method-based interferences can facilitate evaluating inappropriate results of glycated hemoglobin, therefore if HbA1C measurement is inconsistent with clinical presentation, chromatograms should be studied and reported accordingly.
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Pubmed Style
Mehmet Kalaycı, Hakan Ayyıldız, Mahmut Bozkurt, Mustafa Timurkaan. [HbA1C’NİN YÜKSEK PERFORMANSLI LİKİD KROMATOGRAFİ İLE ÖLÇÜMÜNDEKİ İNTERFERANS: HEMOGLOBİN D]. Nobel Med 2016; 12(2): 66-68, Turkish. -
Web Style
Mehmet Kalaycı, Hakan Ayyıldız, Mahmut Bozkurt, Mustafa Timurkaan. [HbA1C’NİN YÜKSEK PERFORMANSLI LİKİD KROMATOGRAFİ İLE ÖLÇÜMÜNDEKİ İNTERFERANS: HEMOGLOBİN D]. www.nobelmedicus.com/en/Article.aspx?m=1716 [Access: Mayıs 24, 2021], Turkish. -
AMA (American Medical Association) Style
Mehmet Kalaycı, Hakan Ayyıldız, Mahmut Bozkurt, Mustafa Timurkaan. [HbA1C’NİN YÜKSEK PERFORMANSLI LİKİD KROMATOGRAFİ İLE ÖLÇÜMÜNDEKİ İNTERFERANS: HEMOGLOBİN D]. Nobel Med 2016; 12(2): 66-68, Turkish. -
Vancouver/ICMJE Style
Mehmet Kalaycı, Hakan Ayyıldız, Mahmut Bozkurt, Mustafa Timurkaan. [HbA1C’NİN YÜKSEK PERFORMANSLI LİKİD KROMATOGRAFİ İLE ÖLÇÜMÜNDEKİ İNTERFERANS: HEMOGLOBİN D]. Nobel Med (2016); 12(2): 66-68, [cited Mayıs 24, 2021], Turkish. -
Harvard Style
Mehmet Kalaycı, Hakan Ayyıldız, Mahmut Bozkurt, Mustafa Timurkaan. (2016) [HbA1C’NİN YÜKSEK PERFORMANSLI LİKİD KROMATOGRAFİ İLE ÖLÇÜMÜNDEKİ İNTERFERANS: HEMOGLOBİN D]. Nobel Med, 12(2): 66-68, Turkish.