Objective: Moringa oleifera plant is used in both traditional medicine and nutrition due to its beneficial content. This study aimed to evaluate the occluding and remineralization effects of Moringa oleifera.

Material and Method: Seventy-five enamel and one- hundred-five dentin samples were prepared. Enamel samples were subjected to demineralization solution (pH=4.5) to create initial caries lesions and divided into five groups (n=15): Control group (no treatment), (S) Sensodyne Repair and Protect, (M) Herbal toothpaste containing Moringa oleifera, (Mff) Herbal toothpaste containing Moringa oleifera with fluoride, and (MO) Moringa oleifera oil. To expose the dentin tubules, the dentin samples were immersed in a 6% citric acid solution for 2 minutes and then divided into seven groups (n=15). Control, (S), (M), (Mff), (MO), (R) R.O.C.S. Sensitive, and (O) Oral-B Sensitivity and Gum Calm. The agents were applied three times daily for seven days using a pH-cycle model. All agents were kept on the surface of samples for 2 minutes and then applied for 1 minute using an electric toothbrush (Oral-B Test Drive (Genius)). Samples were evaluated with microhardness device and fluorescence- based devices (ffluoreCam and DIAGNOdent Pen).

Results: According to fluorescence and microhardness evaluations, the remineralization effect on enamel samples was similar in all treatment groups, except for MO (p<0.001). On dentin samples, after the treatment, microhardness values were significantly lower in the control group following MO (p<0.001). ffluorescence results were significantly higher in the treatment groups compared to the MO and control groups (p<0.001).

Conclusion: Herbal toothpastes containing Moringa oleifera were effective in remineralization and dentin tubule occlusion.

Herbal toothpaste Demineralization Moringa oleifera Remineralization Dentin hypersensitivity