Objective: The aim of this in-vitro study is to evaluate the antibacterial efficacy of different toothpastes on Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Candida albicans, Staphylococcus aureus and Escherichia coli.
Material and Method: Three conventional (Signal Expert Protection, Klorhex Daily Care, Klorhex Intensive Care), two conventional with herbal ingredients (Gumgumix, Colgate Hemp Seed Oil), one experimental based, and six experimental herbal (hemp seed oil, hemp extract, and various metal nanoparticles) toothpastes were tested to determine antimicrobial activity through the agar-well diffusion method.

Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Staphylococcus aureus, Escherichia coli and Candida albicans strains were prepared at 108 CffU/mL in physiological saline, then spread on agar media. Wells with 6 mm diameter were punched and the toothpaste samples were transferred. Paper discs containing 0.2% chlorhexidine digluconate were used as the positive control. After incubation at 37°C for 24-48 hours, the inhibition zones that formed around the wells were measured.

Results: It was observed that Klorhex Intensive Care and one experimental toothpaste (containing Ag nanoparticle, hemp oil, and hemp extract) were effective against all the microorganisms used in the study. The herbal toothpaste that contained ginger didn’t perform any antimicrobial activity against the microorganisms. Signal Expert Protection, Colgate Hemp Seed Oil, and Klorhex Daily Care toothpastes were effective against all of the study’s microorganisms except E. coli.

Conclusion: The addition of hemp seed oil and extract increased antibacterial activity in some experimental toothpastes.

Toothpaste Herbal Hemp Streptococcus mutans Nanoparticles