RESEARCH ARTICLE
Nobel Med 2019; 15(3): 44-51

IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES

Tuğba Kula Atik, Rıza Aytaç Çetinkaya, Mustafa Özyurt, Bülent Atik, Bayhan Bektöre, Burak M. Selek, Ercan Yenilmez, Orhan Baylan
ABSTRACT
Objective: Our study aimed to reveal the OXA-like carbapenemase genes and molecular epidemiological relationship in A. baumannii isolates and compare antibiotic susceptibility status-resistance genes-Pulsed Field Gel Electrophoresis (PFGE) patterns.

Material and Method: A total of 100 A. baumannii isolates obtained from several clinical specimens were examined by VITEK2 automated identification system (Biomerieux, France) for the antibiotic susceptibility profile, by multiplex polymerase chain reaction (PCR) method for OXA-type -lactamases and by PFGE method for the clonal relationship.

Results: 57% of the isolates were obtained from respiratory system specimens. Among all the antibiotics, colistin was the most effective agent with 100% sensitivity, followed by tigecycline with 93%. All of the isolates were resistant to

piperacillin, piperacillin/tazobactam and ciprofloxacin. Resistance against both imipenem and meropenem were detected as 95%. OXA-51 gene was found in all and OXA- 23 gene was found in 92 (92%) of the isolates. None of the isolates had OXA-24 or OXA-58 genes. We identified 19 different clonal clusters among 100 isolates by PFGE method. We revealed that some of the clones were clustered in a certain period of time, and this was supported by the antibiogram results and OXA gene profiling.

Conclusion: Our study identified high rates OXA-23 gene locus positivity, presented the current clonal similarity and time relationship among the clonal clusters. These results emphasize the importance of molecular epidemiological methods as well as standard infection control programs to prevent spreading of A. baumannii.
Keywords
Acinetobacter baumannii Antibiotic resistance Oxa-type carbapenemases Polymerase chain reaction (PCR) Pulsed field gel electrophoresis (PFGE)

Authors Orcid Profiles

0000-0002-2433-1977
0000-0002-6867-0073
0000-0002-6876-2963
0000-0002-1225-7693
0000-0003-0731-2878
0000-0002-5676-9527
0000-0002-1145-8856
0000-0002-6529-7824

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  • Pubmed Style
    Tuğba Kula Atik, Rıza Aytaç Çetinkaya, Mustafa Özyurt, Bülent Atik, Bayhan Bektöre, Burak M. Selek, Ercan Yenilmez, Orhan Baylan. [IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES]. Nobel Med 2019; 15(3): 44-51, English.
  • Web Style
    Tuğba Kula Atik, Rıza Aytaç Çetinkaya, Mustafa Özyurt, Bülent Atik, Bayhan Bektöre, Burak M. Selek, Ercan Yenilmez, Orhan Baylan. [IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES]. www.nobelmedicus.com/en/Article.aspx?m=1716 [Access: Mayıs 24, 2021], English.
  • AMA (American Medical Association) Style
    Tuğba Kula Atik, Rıza Aytaç Çetinkaya, Mustafa Özyurt, Bülent Atik, Bayhan Bektöre, Burak M. Selek, Ercan Yenilmez, Orhan Baylan. [IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES]. Nobel Med 2019; 15(3): 44-51, English.
  • Vancouver/ICMJE Style
    Tuğba Kula Atik, Rıza Aytaç Çetinkaya, Mustafa Özyurt, Bülent Atik, Bayhan Bektöre, Burak M. Selek, Ercan Yenilmez, Orhan Baylan. [IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES]. Nobel Med (2019); 15(3): 44-51, [cited Mayıs 24, 2021], English.
  • Harvard Style
    Tuğba Kula Atik, Rıza Aytaç Çetinkaya, Mustafa Özyurt, Bülent Atik, Bayhan Bektöre, Burak M. Selek, Ercan Yenilmez, Orhan Baylan. (2019) [IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES]. Nobel Med, 15(3): 44-51, English.